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DR1025 Sigma-Aldrich

PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb

liquid, clone, Calbiochem®

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Properties

Related Categories Alphabetical Index, Antibodies, CE-CH, Primary Antibodies
clone   polyclonal
biological source   rabbit
species reactivity   mouse, human, rat, monkey
form   liquid
shipped in   wet ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   -20C

Description

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~56 kDa Chk1 protein when phosphorylated at Ser317.

Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser317 in UV-treated COS cells.

This PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of Chk1 (pSer³¹⁷).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1

Application


Immunoblotting (1:1000)

Immunocytochemistry (1:500, fluorescence)

Paraffin Sections (1:50)

Physical form

In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Reconstitution

Do not aliquot.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

50 μL in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Lysates from COS cells treated with UV

Other Notes

Does not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.

Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol.21, 4129.
Shieh, S.Y., et al. 2000. Genes Dev.14, 289.
Martinho, R.G., et al. 1998. EMBO J.17, 7239.
Zeng, Y., et al. 1998. Nature395, 507.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Protocols & Articles

Articles

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