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DR1047 Sigma-Aldrich

Anti-UHRF1 Mouse mAb (3A11)

liquid, clone 3A11, Calbiochem®

Synonym: Anti-Ubiquitin-Like PHD/RING Finger Domain 1

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, UH-UZ
clone   3A11, monoclonal
biological source   mouse
species reactivity   human
form   liquid
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~90 kDa UHRF1 protein.

Recognizes the ~90 kDa UHRF1 protein in HeLa cells and human endometrium tissue.

This Anti-UHRF1 Mouse mAb (3A11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of UHRF1.

Immunogen

A recombinant polypeptide corresponding to amino acides 694-794 of human rUHRF1, expressed as a GST fusion protein

NA

Application




ELISA (see comments)

Immunoblotting (1-5 µg/ml)

Immunocytochemistry (10 µg/ml)

Paraffin Sections (3 µg/ml)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)

Analysis Note

Positive Control
HeLa cells, Human endometrium tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Mistry H, et al. 2008. Biochem. Biophys. Res. Commun.375 321.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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