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ECM104 Sigma-Aldrich

Millicoat Human Collagen Type I Coated Strips (96-Wells)

96-well plate coated with human Collagen Type I used for cell adhesion studies.

Synonym: Formerly under the CytoMatrix ™ brand name.

  •  eCl@ss 32161000

  •  NACRES NA.75



Quality Level   100
species reactivity   human
mfr. no.   Chemicon®
application(s)   activity assay: suitable
  cell analysis: suitable (cell based assay)
input   sample type neural stem cell(s)
sample type: human embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
NCBI accession no.   NM_000088.3
UniProt accession no.   P02452
detection method   colorimetric
shipped in   wet ice
Gene Information   human ... COL1A1(1277)


General description

Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion and migration of many diverse cell types on various extracellular matrix (ECM) component proteins. Millicoat Cell Adhesion Strips are provided as 12 x 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The wells in rows A - G have been coated with Human Collagen Type I. Row H of each strip is coated with BSA which serves as a negative assay control. Cells are seeded onto the coated substrate. Subsequently, adherent cells are fixed and stained. Relative attachment is determined using absorbance readings.


1 plate in 96 wells

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones


ECM Coating Protocols for Millicell

Coating of membranes and plastic surfaces with extra cellular matrices (ECMs) promotes cell attachment and monolayer formation. We have developed protocols for four types of ECMs on Millicell®-CM ins...
Keywords: Cell attachment, Cell culture, Condensations

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