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ECM506 Sigma-Aldrich

QCM Chemotaxis Cell Migration Assay, 24-well (5 µm), colorimetric

This 3 um QCM Chemotaxis Assay 24-well plate -colorimetric is performed in a Migration Chamber, based on the Boyden chamber principle.

  •  eCl@ss 32161000

  •  NACRES NA.84

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Properties

Quality Level   100
species reactivity (predicted by homology)   all
mfr. no.   Chemicon®
  QCM
application(s)   activity assay: suitable
  cell analysis: suitable (cell based assay)
detection method   colorimetric
shipped in   wet ice

Description

General description

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Introduction
Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation (1, 2). Most cells are sized from 30 to 50 µm can migrate through 3 to 10 µm pore. Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. The Boyden Chamber system uses a hollow plastic chamber, sealed at one end with a porous membrane. This chamber is suspended over a larger well which may contain medium and/or chemoattractants. Cells are placed inside the Chamber and allowed to migrate through the pores, to the other side of the membrane. Migratory cells are then stained and counted. Most migration assays utilize an 8 µm pore size, as this is appropriate for most cell types, e.g. epithelial and fibroblast cells. The Millipore 5 µm QCM Chemotaxis Assay 24-well- colorimetric utilizes a 5 µm pore size, which is appropriate for a subset of fibroblast cells or cancer cells such as NIH-3T3 and MDA-MAB 231 cells. Cells migrated toward chemoattractants were measured by crystal violet, a nucleic dye, and quantified by spectrophotometers. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, and chemokinesis.
The Millipore QCM 5 µm Chemotaxis Assay 24-well-colorimetric provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells.
In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:
• QCM 8µm 24-well Chemotaxis Cell Migration Assays (ECM508, 509)
• QCM 5µm 24-well Chemotaxis Cell Migration Assay- Fluorometric (ECM507)
• QCM 8µm 96-well Chemotaxis Cell Migration Assay (ECM510)
• QCM 5µm 96-well Chemotaxis Cell Migration Assay (ECM512)
• QCM 3µm 96-well Chemotaxis Cell Migration Assay (ECM515)
• QCM 96-well ECMatrix Cell Invasion Assay (ECM555)
• QCM 96-well Collagen-based Cell Invasion Assay (ECM556)

Packaging

1 plate in 24 wells

Application

The Millipore 5 µm QCM Chemotaxis Assay 24-well-colorimetric is performed in a Migration Chamber, based on the Boyden chamber principle. The 5 µm pore size of this assay′s Boyden chambers is appropriate for studying a subset of fibroblast or cancer cell migration. The quantitative nature of this assay is useful for screening of pharmacological agents. Each kit provides sufficient materials for the evaluation of 24 samples.

The Millipore 5 µm QCM Chemotaxis Assay 24-well-colorimetric is intended for research use only; not for diagnostic applications.

Packaging

24 wells

Components

Sterile 5 µm 24-well Cell Migration Plate Assembly: (Part No. 2005708) Two 24-well plates with 12 inserts per plate (24 inserts total/kit).

Cell Stain: (Part No. 90144) one 20 ml.

Extraction buffer: (Part No. 90145) one 20ml.

Cotton Swaps: (Part No. 10202) 50 each.

Forceps: (Part No. 10203) One each.

Storage and Stability

The 5 µm 24-well Cell Migration Plate and forceps are stored at room temperature. Cell stain and extraction buffer can be store at 2° to 8°C up to the expiration date. Do not freeze any components.

Legal Information

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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