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ES-006 Sigma-Aldrich

EmbryoMax® 0.1% Gelatin Solution

The EmbryoMax 0.1% Gelatin Solution is available in a 500 mL format and may be used for routine mouse embryonic stem cell culture applications.

Synonym: porcine gelatin

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Properties

Related Categories Cell Culture, Classic Media and Salts More...
trade name   Embryomax
  Specialty Media
application(s)   Stem Cell Culture
stem cell type   Mouse Embryonic Stem Cells
  Induced Pluripotent Stem Cells
media form   Liquid
material size   500 mL
shipped in   ambient
storage conditions   Store at ambient temperature for upto 2 years

Description

Physical form

EmbryoMax® 0.1% Gelatin Solution. Gelatin is sourced from porcine. Product is ready to use for plating.

Application

Procedure for Coating Multiwell Dishes

1. Add the appropriate amounts of 0.1% Gelatin in Sterile Water to each well of the plate as indicated in the table below:

Wells_Amount/Well
96100 μL
48300 μL
240.5 mL
121.0 mL
61.5-2.0 mL

2. Leave the gelatin solution on the wells for at least 30 minutes at room temperature, with dish lids on.

3. Remove the gelatin solution and discard.

4. Immediately add cells to the dish.

Note: Do not allow the dishes to dry before adding cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Rapid Analysis of Human Adipose-Derived Stem Cells and 3T3-L1 Differentiation Towards Adipocytes

Obesity is a prevalent health risk in many industrialized nations, and its occurrence is closely associated with a number of pathological disorders, such as non-insulin dependent diabetes, hypertensi...
Keywords: Cancer, Cell culture, Diabetes, Microscopy, Obesity

Protocols

Induced Pluripotent Stem Cell Reprogramming Protocols

Introduction Methods     Reprogramming of Human Fibroblasts using Non-Integrating Self-Replicating RNA Vectors     Reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) using STEMCCA Lentiviral...
Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Immunocytochemistry, Polymerase chain reaction, Respiratory, Transduction, Transfection

Related Content

Mouse Embryonic Stem Cell Culture Procedures & Protocols

The development of transgenic and gene knockout technology has provided an effective tool for the analysis of gene function. Critical to this has been the ability to isolate and culture murine embryo...
Keywords: Antibiotics, Apoptosis, Cell culture, Culture media, PAGE

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