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ES009 Sigma-Aldrich

p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble)

500 ml p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble) for ELISA.



Related Categories Biochemicals and Reagents More...
trade name   Chemicon
brand family   Chemicon
application(s)   ELISA
quantitation wavelength   405 nm
material size   500 mL
shipped in   wet ice
storage conditions   Maintain at 2-8°C for up to one year.
color code   Yellow


Physical form

p-NPP, a single component substrate in proprietary stabilizer.


p-Nitrophenyl phosphate (p-NPP) is the customary product utilized for the assay of alkaline phosphatase. It is usually supplied in tablet or powder form and is dissolved in an appropriate buffer immediately prior to assay. Liquid p-NPP concentrates are also available, but must also be diluted in buffer. CHEMICON, Inc. now provides p-NPP stabilized in diethanolamine (DEA) buffer and available for instant use. The DEA buffer contains Mg++ and Zn++ ions providing optimum activation of alkaline phosphatase labeled probes.

This is an excellent substrate for phosphatase based ELISA assays.

It produces a soluble end product which is yellow in color and reads at 405 nm to 410 nm. Both kinetic and end point determinations can be performed.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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