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ES009-M Sigma-Aldrich

Fetal Bovine Serum

US Origin, EmbryoMax ES Cell Qualified FBS, sterile-filtered, suitable for stem cell culture

Synonym: FBS, FCS

  •  eCl@ss 26020100

  •  NACRES NA.75

Purchase

Properties

biological source   bovine fetus
sterility   sterile-filtered
mfr. no.   Embryomax
  Specialty Media
origin   USA origin
application(s)   cell culture | stem cell: suitable
impurities   endotoxin, tested
  mycoplasma, tested
  virus, tested
storage temp.   −20°C

Description

General description

The EmbryoMax ES Cell Qualified FBS is exclusively of US origin. Each lot is sterile filtered and mycoplasma, endotoxin, and virus tested. Additionally, each lot is prescreened for plating efficiency and maintenance of undifferentiated morphology of mouse embryonic stem cells. A Certificate of Analysis is available upon request for each lot of EmbryoMax ES Cell Qualified FBS.
Note: Precipitates may develop upon freezing and/or thawing of this product. If precipitates are present, filter product through a 0.2 micron filter. This occurrence does not impact product performance.

The maintanence, expansion, and assay of many stem cell and precursor cell cultures relies on media rich in a variety of factors, often supplied by fetal bovine serum (FBS).

Quality

Each lot is tested and qualified for use with mouse embryonic stem cells.

Linkage

Replaces: ES-009-D

Physical form

Liquid

Storage and Stability

Store at -20°C.

Analysis Note

• Endotoxin and hemoglobin tested
• Tested for the presence of bacteria, virus, and mycoplasma
• Triple filtered with 0.1 micron membrane under aseptic conditions

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Novel and Rapid Method to Assess Proliferative Properties of Mammalian Cells by Monitoring Cell Size Distributions

Much research has recently focused on elucidating the mechanisms by which eukaryotic cells regulate their size with respect to growth rate and cell cycle stage. These mechanisms are known to differ b...
Keywords: Apoptosis, Cell culture, Cell proliferation, Flow cytometry, High performance liquid chromatography, Sample preparations

Three-dimensional culture and assessment of drug-induced cell death using the CellASIC® ONIX Microfluidic Platform

Cultured cells have proved to be convenient, powerful model systems for elucidating biological processes and for testing the safety, effcacy and mechanism of therapeutic candidates. The main objectiv...
Keywords: Apoptosis, Biological processes, Cell culture, Centrifugation, Microscopy, Transcription

Protocols

A Dynamic Live Cell Assay Platform

Autophagy (self-eating) is a complex cellular process essential for cell survival under stressed conditions. Recent evidence has further suggested that tumor cells use the autophagic pathway to promo...
Keywords: Apoptosis, Autophagy, Building blocks, Cancer, Cell attachment, Cell culture, Cellular processes, Degradations, Diseases, Environmental, Fluorescent microscopy, Gene expression, High performance liquid chromatography, Transduction, Transfection

Establishing a time course for translocation of FOXO4 in live cells using a novel microfluidic culture platform assay

Forkhead box O (FOXO) belongs to the large Forkhead transcription factor family that were first found to be altered in several types of cancers such as alveolar rhabdomyosarcomas and mixed-lineage le...
Keywords: Apoptosis, Autophagy, Cancer, Cell attachment, Cell culture, Diseases, Environmental, Fluorescent microscopy, Growth factors, Metabolites, Microscopy, Transcription

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Mouse Embryonic Stem Cell Culture Procedures & Protocols

The development of transgenic and gene knockout technology has provided an effective tool for the analysis of gene function. Critical to this has been the ability to isolate and culture murine embryo...
Keywords: Antibiotics, Apoptosis, Cell culture, Culture media, PAGE

Mouse Embryonic Stem Cell Culture

Transgenic and gene knockout technologies are powerful tools for studying gene function. A commonly used method for creating transgenic and knockout mice involves the introduction of genetically modi...
Keywords: Amplification, Cell culture, Cell disruption, Gas chromatography, Gene expression, Genetic, Growth factors, Hormones, Polymerase chain reaction, Vitamins

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