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F1904 Sigma-Aldrich

Fibronectin 120 kDa α Chymotryptic Fragment (Cell Attachment region), Human purified

  •  eCl@ss 32160405

  •  NACRES NA.75

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Properties

Quality Level   100
biological source   human
form   lyophilized
mol wt   120 kDa
mfr. no.   Chemicon®
concentration   1 mg/mL
application(s)   cell culture | mammalian: suitable
input   sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
NCBI accession no.   NM_002026.2
  NM_054034.2
  NM_212474.1
  NM_212475.1
  NM_212476.1
  NM_212478.1
  NM_212482.1
UniProt accession no.   P02751
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FN1(2335)

Description

General description

PROPERTIES:

Contains the cell attachment region of the fibronectin molecule.

Product Source: Human plasma cryoprecipitate (plasma fibronectin), assayed negative for hepatitis B virus, hepatitis C Virus (HCV), HIV-1, HIV-2, HTLV-1, HTLV-2 and Treponema pallidum. Handle as if potentially infectious.

Application

F1904 is suitable for use in ELISA and affinity chromatography, and possibly molecular mapping of fibronectin function.

Physical form

Purified protein. Lyophilized in sodium citrate buffer, pH 7.4. Reconstitute with water.

Storage and Stability

Maintain material at -20°C for 3 months or -70°C for six months.

Analysis Note

One major band at 120kDa in SDS-PAGE

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Protocols & Articles

Articles

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

Protocols

ECM Coating Protocols for Millicell

Coating of membranes and plastic surfaces with extra cellular matrices (ECMs) promotes cell attachment and monolayer formation. We have developed protocols for four types of ECMs on Millicell®-CM ins...
Keywords: Cell attachment, Cell culture, Condensations

Peer-Reviewed Papers
15

References

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