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FC014 Sigma-Aldrich

Fibronectin, Bovine

  •  eCl@ss 32160405

  •  NACRES NA.75

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Properties

Quality Level   100
biological source   bovine
assay   ~95% (affinity purified, SDS-PAGE)
form   liquid
mfr. no.   Chemicon®
concentration   1.0 mg/mL
application(s)   cell culture | mammalian: suitable
input   sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type pancreatic stem cell(s)
UniProt accession no.   P02751
  P07589
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FN1(2335)

Description

General description

Bovine fibronectin was purified from bovine plasma over a gelatin agarose column. A single band at 220kDa (reduced) is identified, corresponding to intact fibronectin under reduced conditions.

Product Source: Bovine plasma

Application

ELISA, Ouchterlony and immunoblotting.

Physical form

Liquid in a solution of 2M Urea in 0.05 M Tris pH 7.0.

Storage and Stability

Aliquot and store up to 12 months at -20°C. For short term (1-2 weeks) use, storage at 2-8°C is acceptable. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

Protocols

ECM Coating Protocols for Millicell

Coating of membranes and plastic surfaces with extra cellular matrices (ECMs) promotes cell attachment and monolayer formation. We have developed protocols for four types of ECMs on Millicell®-CM ins...
Keywords: Cell attachment, Cell culture, Condensations

Peer-Reviewed Papers
15

References

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