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  • FCMAB228F - Milli-Mark Anti-mouse CD45R (B220)-FITC Antibody, clone RA3-3A1/6.1

FCMAB228F Sigma-Aldrich

Milli-Mark Anti-mouse CD45R (B220)-FITC Antibody, clone RA3-3A1/6.1

Synonym: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Antibodies, Primary Antibodies More...
application(s)   flow cytometry: suitable
biological source   rat
clone   RA3-3A1/6.1, monoclonal
isotype   IgMκ
mfr. no.   Milli-Mark
species reactivity   mouse
gene symbol   Ly-5
conjugate   FITC conjugate
shipped in   wet ice
Quality Assurance   Evaluated by flow cytometry using mouse splenocytes



Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

144 kDa Calculated

Analysis Note

Mouse splenocytes


Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

General description

The interleukin-2 receptors consist of three polypeptides (α, β, γ), which in different combinations bind IL-2 (Ishii, 1995). The 55 kDa α-chain is called CD25. CD25 is strongly expressed on the surface of activated T cells (Uchiyama, 1981), and is also expressed on B cells stimulated with anti-IgM antibody, and on monocytes/macrophages stimulated with lipopolysaccharide (Sugamura, 1997). In normal peripheral blood a variable expression on lymphoid cells (about 3-25%) is observed. The specificity of Anti-CD25, ACT-1, is identical to that of CD25-clustered antibodies (Schwarting, 1987; Aziz, 1996).


Animals were immunized with the mouse cell line RAW 112. Spleen cells were fused with the S194/5.XX0.BU.1 myeloma.


Antibody recognizes CD45 isoform 1 also known as B220.

Physical form

Ion Exchange

Purified rat monoclonal IgM conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis

Protocols & Articles
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