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  • FCMAB412F - Milli-Mark Anti-Collagen Type I-FITC Antibody, clone 5D8-G9

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FCMAB412F Sigma-Aldrich

Milli-Mark Anti-Collagen Type I-FITC Antibody, clone 5D8-G9

clone 5D8-G9, Milli-Mark®, from mouse

Synonym: Alpha-1 type I collagen

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CO-CP, Primary Antibodies
conjugate   FITC conjugate
clone   5D8-G9, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
species reactivity   human, human, human
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Milli-Mark®
NCBI accession no.   NP_000079
UniProt accession no.   P02452
Gene Information   human ... COL1A1(1277)

Description

General description

Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.

Specificity

Antibody recognizes Human Collagen Type I.

Immunogen

Purified human type I collagen emulsified in Freund′s complete adjuvant.

Application

Milli-Mark Anti-Collagen Type I-FITC Antibody, clone 5D8-G9 is an antibody against Collagen Type I for use in FC.

Research Category
Cell Structure

Target description

139 kDa Calculated

Physical form

Protein A

Purified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by flow cytometry using HEK293T cells.

Analysis Note

Control
HEK293T cells

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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