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GF156 Sigma-Aldrich

B18R, Human Recombinant Carrier-Free

B18R protein can be used with the Simplicon® RNA Reprogramming Technology (Catalog No. SCR550) to create “foot-print” free human iPS cells at the highest efficiency without unwanted RNA degradation caused by cellular IFN responses.

  •  eCl@ss 32160405

  •  NACRES NA.75

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Properties

Quality Level   100
biological source   human
assay   >98% (SDS-PAGE)
mol wt   Mw 38,389 Da
concentration   0.5 mg/mL
application(s)   cell analysis: suitable (cell culture)
  cell culture | stem cell: suitable
impurities   <0.05 ng/μg cytokine (LAL test)
input   sample type induced pluripotent stem cell(s)
shipped in   dry ice

Description

General description

B18R is a virally-encoded protein that acts as a decoy receptor for Type 1 Interferons. B18R enables increased cell viability during RNA transfection protocols designed to convert human somatic donor cells into iPSCs via direct delivery of synthetic mRNA′s. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta.

Product Source: Insect cells infected with baculovirus: amino acids His20-Glu 351 with a His-tag attached of Vaccinia Virus B18R.

Application

Research Category
Stem Cell Research

Physical form

Sterile liquid;10 mM phosphate, 0.5 M NaCl, pH 7.5 buffer and is filtered with a 0.2µm filter before vialing.

Storage and Stability

For best recovery, quick-spin vial prior to opening. Thaw on ice. While on ice, aliquot B18R protein into sterile, nuclease-free, low protein-binding eppendorf tubes and store at -80°C. Limit repeated freeze and thaw cycle. Use in a sterile environment.
Further dilutions should be made into appropriate buffer or medium.

Analysis Note

The DTT-reduced protein migrates as a 48 kDa polypeptide on SDS-PAGE due to glycosylation. The non-reduced protein migrates as a 46 kDa polypeptide on SDS-PAGE due to internal cystines.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Simplicon™ In Vivo Protein Expression System

Simplicon™ is a novel system that generates immediate, high-sustained protein expression of multiple genes into transfected human cells without the risk of genome integration. The technology employs ...
Keywords: Cloning, Culture media, Gene expression, Genetic, Microscopy, Transfection

Simplicon™ RNA Reprogramming

A ready source of induced pluripotent stem cells (iPSCs) is critical to the effective study of differentiation pathways and the investigation of the therapeutic potential of iPS cells. Since the disc...
Min Lu1, Naohisa Yoshioka2, Steven Dowdy2 and Vi Chu1*

1EMD Millipore, Bioscience Division, Temecula, CA, USA
2University of California, San Diego, La Jolla, CA, USA
Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Microscopy, Respiratory, Transfection

Protocols

Induced Pluripotent Stem Cell Reprogramming Protocols

Introduction Methods     Reprogramming of Human Fibroblasts using Non-Integrating Self-Replicating RNA Vectors     Reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) using STEMCCA Lentiviral...
Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Immunocytochemistry, Polymerase chain reaction, Respiratory, Transduction, Transfection

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