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GF175 Sigma-Aldrich

WNT-1, Human Recombinant

Synonym: WNT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog

  •  eCl@ss 32160405

  •  NACRES NA.75

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Properties

Related Categories Biochemicals and Reagents, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors,
packaging   10 µg (GF175)
UniProt accession no.   P04628
application(s)   stem cell: suitable
  cell culture
assay   >98% (SDS-PAGE and HPLC)
impurities   <0.1 μg/mg protein endotoxin (<1EU/μg)
shipped in   dry ice

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Application

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

General description

Product Source: E. coli.

Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, β-catenin. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.

Quality

Bioactivity assay: The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.

Specificity

Cross Reactivty
Mouse

Physical form

Product is filtered through a 0.2 micron filter before lyophilization. Product is presented in .

Storage and Stability

Store at -20°C for up to 6 months from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Wnt/β-Catenin Signaling

Background Wnt Signaling Components    a) Wnt protein      b) Receptors of Wnt proteins    c) Wnt signaling modulators Wnt /beta-catenin signaling mechanism    a) Wnt signaling – Off    b) Wnt signal...
Keywords: Adhesion, Apoptosis, Cancer, Cell division, Cell proliferation, Clinical, Degradations, Gene expression, Hormones, Ligands, Metabolism, Phosphorylations, Transcription, Transduction, Ubiquitinations

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