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GR01L Sigma-Aldrich

Anti-EGFR (Ab-1) Mouse mAb (528)

lyophilized, clone 528, Calbiochem®

Synonym: Anti-Epidermal Growth Factor Receptor

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Properties

Related Categories Alphabetical Index, Antibodies, E-EI, Primary Antibodies
clone   528, monoclonal
biological source   mouse
species reactivity   human
should not react with   rat, mouse
form   lyophilized
shipped in   ambient
isotype   IgG2a
Quality Level   100
antibody product type   primary antibodies
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C

Description

General description

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.

Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa EGFR protein.

Recognizes the EGF receptor protein in A431 cells and normal skin.

Immunogen

partially purified, human EGFR from human A431 cells

Application

Flow Cytometry (5 µg/ml)

Frozen Sections (2.5 µg/ml)

Immunofluorescence (5 µg/ml)

Immunoprecipitation (1 µg/sample)

Neutralization Studies (see comments and application references)

Paraffin Sections (see application references)

Physical form

Lyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.

Reconstitution

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.

Packaging

100 μg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
A-431 cells or skin tissue

Other Notes

Hunter T. 1984. Nature311, 414.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

This antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.

Safety & Documentation

Safety Information

WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

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