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HCD8MAG-15K Millipore

MILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

  •  eCl@ss 32161000


To order a Milliplex kit, please search for your analyte of interest.




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Properties

Quality Level   200
species reactivity   human
mfr. no.   MILLIPLEX®
assay range   standard curve range: 0.4-1,500 pg/mL
(IL-6)
  standard curve range: 0.5-2,000 pg/mL
(TNFα)
  standard curve range: 1-3,500 pg/mL
(MIP-1α)
  standard curve range: 1-5,000 pg/mL
(Granzyme B)
  standard curve range: 1-5,000 pg/mL
(IFNγ)
  standard curve range: 1-5,000 pg/mL
(IL-5)
  standard curve range: 10-50,000 pg/mL
(Perforin)
  standard curve range: 10-50,000 pg/mL
(sFasL)
  standard curve range: 2-10,000 pg/mL
(IL-4)
  standard curve range: 2-10,000 pg/mL
(sCD137)
  standard curve range: 2-7,500 pg/mL
(IL-13)
  standard curve range: 2-7,500 pg/mL
(IL-2)
  standard curve range: 20-100,000 pg/mL
(Granzyme A)
  standard curve range: 4-15,000 pg/mL
(GM-CSF)
  standard curve range: 400-1,650,000 pg/mL
(sFas)
  standard curve range: 5-20,000 pg/mL
(IL-10)
  standard curve range: 7-30,000 pg/mL
(MIP-1β)
application(s)   multiplexing: suitable
detection method   fluorescent (Luminex xMAP)
shipped in   wet ice

Description

General description

CD8+ T cells (also known as cytotoxic T cells, cytolytic T cells and killer T cells) belong to a sub-group of T cells capable of inducing the death of infected somatic or tumor cells. T cells that bind weakly to Major Histocompatibility Complex (MHC) self-antigens are positively selected into single-positive CD4+ or CD8+ T cells in the thymus. Those CD8+ T cells that survive and mature after activation become cytotoxic cells, expressing T-cell receptors (TCRs) capable of recognizing specific antigenic peptides bound to Class I MHC molecules and the glycoprotein CD8. Affinity between the CD8 protein and the MHC molecule helps to keep the cytotoxic T cell and target closely bound during antigen specific activation. Once activated, CD8+ T cells are generally classified as having a predefined cytotoxic role within the immune system and undergo IL-2 induced clonal expansion, which increases the number of cells specific for the target antigen. The CD8+ T cells then travel throughout the body in search of antigen-positive somatic/tumor cells.

Panel Type: MAGNETIC Cytokines/Chemokines

The MILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel enables you to focus on the therapeutic potential of Human CD8+ T Cell analytes. Coupled with the Luminex® xMAP platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of several analytes simultaneously, which can dramatically improve productivity. The MILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel is the most versatile system available for Mouse CD8+ T Cell research.

MILLIPLEX MAP offers you the ability to:

• Select a 17-plex premixed option or
• Choose any combination of analytes from our panel of 17 analytes to design a custom kit that better meets your needs.
• A convenient “all-in-one” box format gives you the assurance that you will have all the necessary reagents you need to run your assay.

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

• This is an overnight incubation assay requiring 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of all or any combination of the Human CD8+ T-Cell Panel analytes in serum, plasma or tissue/cell lysate and culture supernatant.

Research Category
Inflammation & Immunology

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

Used to detect/quantify: GM-CSF

sCD137

IFNγ

sFas

sFasL

Granzyme A

Granzyme B

IL-2

IL-4

IL-5

IL-6

IL-10

IL-13

MIP-1α

MIP-1β

TNF-α

Perforin

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
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