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HCYP4MAG-64K Millipore

MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel IV

The Human Inflammation / Immunology Bead-Based Multiplex Cytokine/Chemokine Assay kit IV, using the Luminex xMAP technology, enables the simultaneous analysis of 21 cytokine and chemokine biomarkers in human serum, plasma and cell culture samples.

  •  eCl@ss 32161000


To order a Milliplex kit, please search for your analyte of interest.




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Properties

Quality Level   200
species reactivity   human
mfr. no.   MILLIPLEX®
assay range   accuracy: 101%
(BAFF/Blys)
  accuracy: 101%
(BRAK/CSCL14)
  accuracy: 101%
(IL-28B)
  accuracy: 102%
(MPIF-1/CL23)
  accuracy: 106%
(IL-19)
  accuracy: 110%
(HCC-4/CCL16)
  accuracy: 110%
(IL-34)
  accuracy: 89%
(IL-32α)
  accuracy: 95%
(IL-35)
  accuracy: 99%
(IL-37)
  sensitivity: 0.30 ng/mL
(YKL40/CHI3L1)
  sensitivity: 0.8 ng/mL
(IL-24)
  sensitivity: 0.8 ng/mL
(IL-35)
  sensitivity: 0.9 ng/mL
(BAFF/BLyS)
  sensitivity: 10.5 ng/mL
(HMGB1/HMG1)
  sensitivity: 100.8 pg/mL
(CCL28)
  sensitivity: 121.5 pg/mL
(IL-34)
  sensitivity: 17.2 pg/mL
(IL-28B/IFN-λ3)
  sensitivity: 248.2 pg/mL
  sensitivity: 27.7 pg/mL
(IL-14/α-Taxilin)
  sensitivity: 28.4 pg/mL
(HCC-4)
  sensitivity: 3.5 pg/mL
(IL-36β)
  sensitivity: 32.1 pg/mL
(IL-32α)
  sensitivity: 33.2 pg/mL
(IL-38)
  sensitivity: 375.6 pg/mL
(IFNβ)
  sensitivity: 4.7 pg/mL
(CXCL16)
  sensitivity: 5.2 pg/mL
(IL-37/IL-1F7)
  sensitivity: 6.6 pg/mL
(MIP-4/PARC)
  sensitivity: 607 pg/mL
(BRAK)
  sensitivity: 7.8 pg/mL
(MPIF-1)
  standard curve range: 0.1-100 ng/mL
(YKL40/CHI3L1)
  standard curve range: 0.44-400 ng/mL
(BAFF/Blys)
  standard curve range: 0.7-750 ng/mL
(IL-24)
  standard curve range: 0.8-800 ng/mL
(IL-35)
  standard curve range: 10-10,000 pg/mL
(MPIF-1/CL23)
  standard curve range: 2-2,000 pg/mL
(IL-36β)
  standard curve range: 2.0-2,000 ng/mL
(HMGB1)
  standard curve range: 24-25,000 pg/mL
(IL-14)
  standard curve range: 244-250,000 pg/mL
(IL-19)
  standard curve range: 29-30,000 pg/mL
(IL-28B)
  standard curve range: 3-3,000 pg/mL
(CXCL16)
  standard curve range: 39-40,000 pg/mL
(IL-32α)
  standard curve range: 488-500,000 pg/mL
(BRAK/CXCL14)
  standard curve range: 488-500,000 pg/mL
(IFNβ)
  standard curve range: 49-50,000 pg/mL
(IL-38)
  standard curve range: 5-5,000 pg/mL
(IL-37)
  standard curve range: 5-5,000 pg/mL
(MIP-4/PARC/CCL18)
  standard curve range: 98-100,000 pg/mL
(CCL28)
  standard curve range: 98-100,000 pg/mL
(IL-34)
application(s)   multiplexing: suitable
detection method   fluorescent (Luminex xMAP)
shipped in   ambient

Description

General description

EMD Millipores MILLIPLEX® MAP Cytokine/Chemokine Magnetic Bead Panel IV is a 20-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma or culture supernatant samples: BAFF/Blys, BRAK/CSCL14, CCL28, CXCL16, HMGB1, IFNβ, IL-14, IL-19, IL-24, IL-28B, IL-32α, IL-34, IL-35, IL-36β, IL-37, IL-38, MIP-4/PARC/CCL18, MPIF-1/CL23 and YKL40/CHI3L1.

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® MAP panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment
• Detection antibody cocktails designed to yield consistent analyte profiles within panel

EMD Millipores MILLIPLEX® MAP Human Cytokine IV Magnetic Bead Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.

Inflammation and immune cell response are key to maintaining homeostasis. The more we understand mechanisms of disease, the more we discover the role that inflammation plays in the etiology of disease: certainly immunological diseases, such as allergic reactions, asthma, infectious disease and an ever-growing number of conditions under the autoimmune umbrella, but also neurodegenerative disease, metabolic and cardiovascular disease and even cancer. The ability to measure multiple inflammation biomarkers simultaneously using EMD Millipore’s MILLIPLEX MAP panels, based on the Luminex xMAP technology, to establish disease profiles that can be used for screening, diagnosis and response to treatment is critical for understanding the pathogenesis of these diseases.

Panel Type: MAGNETIC Cytokines/Chemokines

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.

• A maximum of 25 μL per well of 1:4 diluted serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay. Please note that HMGB1 can be measured in serum and plasma samples only.

Research Category
Inflammation & Immunology

Used to detect/quantify:

BAFF/Blys

BRAK/CXCL14

CCL28

CXCL16

HCC-4/CCL16

HMGB1

IFNβ

IL-14

IL-19

IL-24

IL-28B

IL-32α

IL-34

IL-35

IL-36β/IL-1F8

IL-37/IL-1F7

IL-38/IL-1F10

MIP-4

MPIF-1/CCL23

YKL40

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96 well plate

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please note: Sample Type must be selected when configuring this kit. HMGB1 can be measured in serum and plasma samples only.

Legal Information

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
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