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IEVH85R Millipore

Immobilon-E PVDF Membrane

The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.

  •  eCl@ss 32031613

  •  NACRES NB.23

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Properties

Related Categories Membranes & Blotting Paper, Millipore PVDF Membranes for Western Blotting, Molecular Biology, Protein Electrophoresis, Proteomics,
material   PVDF membrane
  plain filter
  white filter
feature   easily wetted with water
mfr. no.   Immobilon®
application(s)   western blot: suitable
filter L × W   8.5 cm × 10 m
pore size   0.45 μm pore size
compatibility   for use with Amido black
  for use with Colloidal gold
  for use with Coomassie brilliant blue
  for use with India ink
  for use with Ponceau-S red
  for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
  for use with Transillumination
detection method   chemiluminescent
  colorimetric
  radioactive
shipped in   ambient

Description

General description

Filter Code: IEVH

Immobilon-E is the first PVDF membrane for Western blotting that can be wetted with with aqueous transfer buffer. This eliminates the alcohol pre-wet step required with other PVDF membranes.

Primary Binding Mechanisms: Electrostatic, hydrophobic

This 8.5cm x 10m roll offers 1-cut convenience for standard mini-blots (7 x 8.5cm) and midi-blots (8.5 x 13.5cm). It works with the optional Immobilon NOW Dispenser (catalog number IMDISP).

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most Western blotting applications, especially proteins greater than 20 kDa
- Compatible with chemiluminescent and chromogenic detection

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes.

Features & Benefits:
- High protein binding provides stronger signal
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be stripped and reprobed multiple times

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Immobilon® NOW Transfer Membrane rolls for Western blotting

Immobilon® NOW rolls offer the convenience of pre-cut sheets with the flexibility of rolls in the formats you depend on: Immobilon®-E, Immobilon®-P, Immobilon®-FL and Immobilon®-PSQ membranes.
Keywords: Dot blot, Western blot

Immobilon®-E PVDF membrane for Western blotting

Immobilon®-E provides the first PVDF blotting membrane that wets in transfer buffer or water. It removes the alcohol pre-wet step while maintaining the high performance associated with Immobilon® tra...
Keywords: Western blot

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

Figure 1. Structure of glycosylated human glutaminyl cyclase (PDB 3SI0), NGL Viewer (AS Rose et al. (2018) NGL viewer: web-based molecular graphics for large complexes. Bioinformatics doi:10.1093/bio...
Keywords: Buffers, Catalysis, Cell disruption, Degradations, Digestions, Electrophoresis, Gel electrophoresis, Glycosylations, Homogenization, N-linked glycosylation, O-linked glycosylation, PAGE, Phosphorylations, Sample preparations, Separation, Ubiquitinations, Western blot

Protocols

Stripping and Reprobing Western Blotting Membranes

Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are separated by SDS-PAGE and transferred to a nitrocellulose or PVDF membrane...
Keywords: Buffers, Detergents, Immobilization, PAGE, Western blot

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Immobilon® Now 預先裁切轉漬膜

Immobilon ® NOW限時優惠    ● 各種材質任選「買四送一」    ● 最常使用的8.5cm黃金size    ● 體積小易收納、避免剩材浪費              
Keywords: Detection methods, Western blot

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