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  • IHCR1001-6 - Anti-Neuron-Specific Nuclear Protein Antibody, prediluted, clone A60

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IHCR1001-6 Sigma-Aldrich

Anti-Neuron-Specific Nuclear Protein Antibody, prediluted, clone A60

clone A60, Chemicon®, from mouse

Synonym: NeuN

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, NE-NI, Primary Antibodies
clone   A60, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable (paraffin)
species reactivity   ferret, avian, chicken, mouse, rat, pig, human, vertebrates, salamander, primate
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®

Description

General description

NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).

Specificity

neuron-specific protein NeuN

Immunogen

Purified cell nuclei from mouse brain.

Application

Anti-Neuron-Specific Nuclear Protein Antibody, prediluted, clone A60 detects level of Neuron-Specific Nuclear Protein & has been published & validated for use in IH(P).

Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

Incubation: 30 minutes with IHC Select Detection Kits.

NeuN, has been prediluted for use as the primary antibody with Chemicon′s IHC Select Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier′s IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR1001-6.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Physical form

Format: Purified

Liquid diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% as preservative.

Storage and Stability

Maintain at 2-8°C. Refer to vial for expiration dating.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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