• USA Home
  • IHCR2014-6 - IHC Select Anti-Neuron-Specific Enolase Antibody, prediluted, clone BBS/NC/VI-H14

EMAIL THIS PAGE TO A FRIEND
IHCR2014-6 Sigma-Aldrich

IHC Select Anti-Neuron-Specific Enolase Antibody, prediluted, clone BBS/NC/VI-H14

clone BBS/NC/VI-H14, from mouse

Synonym: NSE

  •  eCl@ss 32160702

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, NE-NI, Primary Antibodies
clone   BBS/NC/VI-H14, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable (paraffin)
species reactivity   human
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
  IHC Select
NCBI accession no.   NM_001975.2
UniProt accession no.   P09104

Description

Specificity

Reacts with human gamma-gamma enolase (Mr 46000). This antibody does not cross react with alpha or beta subunits of NSE.

Immunogen

Gamma-gamma enolase purified from human brain

Application

Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

Incubation: 10 minutes with IHC Select Detection Kits.

NSE has been prediluted for use as the primary antibody with Chemicon′s IHC Select Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier′s IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR2014-6.

This IHC Select Anti-Neuron-Specific Enolase Antibody, prediluted, clone BBS/NC/VI-H14 is validated for use in IH(P) for the detection of Neuron-Specific Enolase.

Physical form

Format: Purified

Monoclonal mouse antibody supplied in liquid format as tissue culture supernatant, diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% Kathon as a preservative.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?