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  • IPFL85R - Immobilon-FL PVDF, 0.45 µm, 8.5 cm x 10 m roll

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IPFL85R Millipore

Immobilon-FL PVDF, 0.45 µm, 8.5 cm x 10 m roll

0.45 um ( 0.45 micron filter ) pore size Hydrophobic PVDF Transfer Membrane for western blotting using Fluorescent, Chemifluorescence, Chromogenic, or Chemiluminescence. Comes in a 8.5 cm x 10 m roll.

  •  eCl@ss 32031613

  •  NACRES NB.23

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Properties

Related Categories Membranes & Blotting Paper, Millipore PVDF Membranes for Western Blotting, Molecular Biology, Protein Electrophoresis, Proteomics,
material   PVDF membrane
  plain filter
  white filter
feature   hydrophobic
mfr. no.   Immobilon®
application(s)   western blot: suitable
filter L × W   8.5 cm × 10 m
pore size   0.45 μm pore size
capacity   155 μg/cm2 adsorption capacity (insulin)
  205 μg/cm2 adsorption capacity (BSA)
  300 μg/cm2 adsorption capacity (goat IgG)
compatibility   for use with All fluorescence dyes
  for use with Amido black
  for use with Coomassie brilliant blue
  for use with Ponceau-S red
  for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
detection method   chemiluminescent
  colorimetric
  fluorescent
shipped in   ambient

Description

General description

Filter Code: IPFL

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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Immobilon®-E PVDF membrane for Western blotting

Immobilon®-E provides the first PVDF blotting membrane that wets in transfer buffer or water. It removes the alcohol pre-wet step while maintaining the high performance associated with Immobilon® tra...
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Immobilon®-FL PVDF membrane for Western blotting

The Immobilon®-FL PVDF transfer membrane (0.45 μm) is the first transfer membrane specifically optimized for fluorescence-based detection of Western blots. Its extremely low background autofluorescen...
Keywords: Adsorption

Immobilon®-P PVDF membrane for Western blotting

Immobilon®-P is the original, and most commonly used, PVDF transfer membrane for Western blotting. Available in sheets, rolls, and pre-cut Western blotting sandwiches.
Keywords: Adsorption, Western blot

Immobilon®-PSQ PVDF membrane for Western blotting

Immobilon®-PSQ transfer membrane for smaller proteins. This PVDF membrane has a 0.2 μm pore size with a thickness of ~200 μm. Because it has smaller pores and approximately three times the internal s...

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

Figure 1. Structure of glycosylated human glutaminyl cyclase (PDB 3SI0), NGL Viewer (AS Rose et al. (2018) NGL viewer: web-based molecular graphics for large complexes. Bioinformatics doi:10.1093/bio...
Keywords: Buffers, Catalysis, Cell disruption, Degradations, Digestions, Electrophoresis, Gel electrophoresis, Glycosylations, Homogenization, N-linked glycosylation, O-linked glycosylation, PAGE, Phosphorylations, Sample preparations, Separation, Ubiquitinations, Western blot

Protocols

Stripping and Reprobing Western Blotting Membranes

Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are separated by SDS-PAGE and transferred to a nitrocellulose or PVDF membrane...
Keywords: Buffers, Detergents, Immobilization, PAGE, Western blot

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