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IPVH08100 Millipore

Immobilon-P PVDF Membrane

10 sheets, 8 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

  •  eCl@ss 32031602

  •  NACRES NB.22

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Properties

Related Categories Membranes & Blotting Paper, Millipore PVDF Membranes for Western Blotting, Molecular Biology, Protein Electrophoresis, Proteomics,
Quality Level   400
material   PVDF membrane
  plain filter
  white filter
feature   hydrophobic
mfr. no.   Immobilon®
application(s)   western blot: suitable
filter L × W   8 cm × 10 cm
pore size   0.45 μm pore size
capacity   160 μg/cm2 adsorption capacity (insulin)
  215 μg/cm2 adsorption capacity (BSA)
  294 μg/cm2 adsorption capacity (goat IgG)
compatibility   for use with Amido black
  for use with CPTS
  for use with Colloidal gold
  for use with Coomassie brilliant blue
  for use with India ink
  for use with Ponceau-S red
  for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
  for use with Toluidine blue
  for use with Transillumination
detection method   chemiluminescent
  colorimetric
  fluorescent
  radioactive
shipped in   ambient

Description

General description

Filter Code: IPVH

Overview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore′s Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times

Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths

Primary Binding Mechanisms: Electrostatic, hydrophobic

Application

10 sheets, 8 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Immobilon®-P PVDF membrane for Western blotting

Immobilon®-P is the original, and most commonly used, PVDF transfer membrane for Western blotting. Available in sheets, rolls, and pre-cut Western blotting sandwiches.
Keywords: Adsorption, Western blot

Protocols

Recipe Calculators for Western Blotting Buffers & Solutions

The recipes listed enable the accurate preparation of commonly used solutions for Western blotting, whether you are mixing low volumes for a few experiments or multiple liters for the entire lab to u...
Keywords: Buffers, Detergents, Western blot

Peer-Reviewed Papers
15

References

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