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  • IPVH85R - Immobilon-P Membrane, PVDF, 0.45 µm, 8.5 cm x 10 m roll

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IPVH85R Millipore

Immobilon-P Membrane, PVDF, 0.45 µm, 8.5 cm x 10 m roll

0.45 um ( 0.45 micron filter ) pore size Hydrophobic immobilon-P PVDF Transfer Membrane for western blotting using Chemiluminescence, Chromogenic or Radioactive detection methods. Comes in an 8.5 cm x 10 m roll.

  •  eCl@ss 32031613

  •  NACRES NB.23

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Properties

Related Categories Membranes & Blotting Paper, Millipore PVDF Membranes for Western Blotting, Molecular Biology, Protein Electrophoresis, Proteomics,
material   PVDF membrane
  plain filter
  white filter
feature   hydrophobic
mfr. no.   Immobilon®
application(s)   western blot: suitable
filter L × W   8.5 cm × 10 m
pore size   0.45 μm pore size
capacity   160 μg/cm2 adsorption capacity (insulin)
  215 μg/cm2 adsorption capacity (BSA)
  294 μg/cm2 adsorption capacity (goat IgG)
compatibility   for use with Amido black
  for use with CPTS
  for use with Colloidal gold
  for use with Coomassie brilliant blue
  for use with India ink
  for use with Ponceau-S red
  for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
  for use with Toluidine blue
  for use with Transillumination
detection method   chemiluminescent
  colorimetric
  radioactive
shipped in   ambient

Description

General description

Filter Code: IPVH

Primary Binding Mechanisms: Electrostatic, hydrophobic

Linkage

Replaces: INCP00010

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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Immobilon®-P PVDF membrane for Western blotting

Immobilon®-P is the original, and most commonly used, PVDF transfer membrane for Western blotting. Available in sheets, rolls, and pre-cut Western blotting sandwiches.
Keywords: Adsorption, Western blot

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

Figure 1. Structure of glycosylated human glutaminyl cyclase (PDB 3SI0), NGL Viewer (AS Rose et al. (2018) NGL viewer: web-based molecular graphics for large complexes. Bioinformatics doi:10.1093/bio...
Keywords: Buffers, Catalysis, Cell disruption, Degradations, Digestions, Electrophoresis, Gel electrophoresis, Glycosylations, Homogenization, N-linked glycosylation, O-linked glycosylation, PAGE, Phosphorylations, Sample preparations, Separation, Ubiquitinations, Western blot

Protocols

Stripping and Reprobing Western Blotting Membranes

Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are separated by SDS-PAGE and transferred to a nitrocellulose or PVDF membrane...
Keywords: Buffers, Detergents, Immobilization, PAGE, Western blot

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