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MAB1072 Sigma-Aldrich

Anti-Erythropoietin Antibody, clone 1C8

clone 1C8, Chemicon®, from mouse

Synonym: erythropoietin, Epoetin, epoetin

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Antibodies for Cell Biology, Antibodies for Neurobiology, Antibodies to Chemokines, Antibodies to Cytokines and Growth Factors,
Quality Level   100
biological source   mouse
clone   1C8, monoclonal
species reactivity   human
mfr. no.   Chemicon®
application(s)   ELISA: suitable
  immunofluorescence: suitable
  western blot: suitable
isotype   IgG2b
NCBI accession no.   NM_000799.2
UniProt accession no.   P01588
shipped in   wet ice

Description

General description

Erythropoietin (Epo) is a glycoprotein consisting of one 165-amino acid single-chain polypeptide with two internal disulfide bonds. When fully glycosylated, the molecular weight of Epo is approximately 34 kDa. Normally produced by the liver and kidney, Epo is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia.

Specificity

Reacts with human Erythropoietin (Epo).

Immunogen

Recombinant human Epo.

Application

Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.

ELISA:
For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.

Immunosorbent preparation

Optimal working dilutions must be determined by the end user.

Research Category
Metabolism

Research Sub Category
Metabolic Hormones & Receptors

Quality

Evaluated by Western Blot on Mouse kidney lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.

Target description

34 kDa (glycosylated)

Physical form

Format: Purified

Protein A Purfied

Purified mouse monoclonal IgG2b in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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