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  • MAB1304 - Mouse Anti-Human IgG Antibody, Fd, clone HP6045

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MAB1304 Sigma-Aldrich

Mouse Anti-Human IgG Antibody, Fd, clone HP6045

clone HP6045, Chemicon®, from mouse

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, Human IgG Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies,
Quality Level   100
biological source   mouse
antibody product type   secondary antibodies
clone   HP6045, monoclonal
species reactivity   human
mfr. no.   Chemicon®
application(s)   ELISA: suitable
  radioimmunoassay: suitable
  western blot: suitable
isotype   IgG1
shipped in   wet ice

Description

Specificity

Human IgG Fd.

ELISA Specificity: (Hamilton et al., 1987)

human IgG subclass 1 100%

human IgG subclass 2 100%

human IgG subclass 3 100%

human IgG subclass 4 99%

human IgG Pan 100%

human IgG Fc 0.04%

human IgG Fab 100%



Isoelectric Focusing: mean pI 6.5

Immunogen

Epitope: Fd region

Application

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

This Mouse anti-Human IgG Antibody, Fd, clone HP6045 is validated for use in ELISA, RIA, WB for the detection of Human IgG.

Useful as both a capture antibody and a detection antibody for human IgG Fd (Papadea, C., et al., Clin. Chem. (1985). 31(12):1940-1945; Hamilton, R.G., et al., Electrophoresis (1987) 8:127-134).

ELISA

Radioimmunoassay (RIA)

Western blotting.

Final working dilutions must be determined by end user.

Physical form

Format: Purified

Liquid in 0.02 M phosphate buffer with 0.25 M NaCl and 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to six months after date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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