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  • MAB1308 - Mouse Anti-Human IgG2 Antibody, clone HP6002, Fc

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MAB1308 Sigma-Aldrich

Mouse Anti-Human IgG2 Antibody, clone HP6002, Fc

clone HP6002, Chemicon®, from mouse

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, Human IgG Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies,
clone   HP6002, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  radioimmunoassay: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   secondary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_001002273.1
  NM_001002274.1
  NM_001002275.1
  NM_004001.3
UniProt accession no.   P31994
Gene Information   human ... FCGR2B(2213)

Description

Specificity

Human IgG2 Fc. ELISA Specificity: (Hamilton et al., 1987) human IgG subclass 1-0.02% human IgG subclass 2- 100% human IgG subclass 3- 0.01%

human IgG subclass 4- 0.01% Isoelectric Focusing: mean pI 7.1

Application

This Mouse anti-Human IgG2 Antibody, clone HP6002, Fc is validated for use in ELISA, RIA, WB for the detection of Human IgG2.

This antibody employed as both a capture antibody and a detection antibody for human IgG2 in enzyme linked immunosorbent assays, radio-immunoassay and EIA affinity immunoblots (Papadea et al., 1985; Hamilton, et al., 1987).

Final working dilutions must be determined by end user.

Physical form

Format: Purified

Purified Immunoglobulin. Liquid in 0.02M Phosphate buffer, pH=7.6, 0.25M NaCl containing 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C for up to one year in undiluted aliquots.

Linkage

Replaces: 411462-200UG

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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