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  • MAB1334 - Anti-Collagen Types I, II & III Antibody, clone MMCHABC

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MAB1334 Sigma-Aldrich

Anti-Collagen Types I, II & III Antibody, clone MMCHABC

clone MMCHABC, Chemicon®, from mouse

Synonym: Interstitial Collagens

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CO-CP, Primary Antibodies
clone   MMCHABC, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunohistochemistry: suitable
  radioimmunoassay: suitable
species reactivity   human
shipped in   dry ice
isotype   IgM
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
suitability   not suitable for Western blot
NCBI accession no.   NM_000088.3
UniProt accession no.   P02452
  P02458
  P02461
Gene Information   human ... COL1A1(1277)

Description

Specificity

Human collagen types I, II and III (both native and denatured). No cross reactivity with human plasma proteins, other collagen types or non-collagenous proteins in ELISA, RIA or immunoblot.

Immunogen

Human collagen type I

Application

Anti-Collagen Types I, II & III Antibody, clone MMCHABC detects level of Collagen Types I & has been published & validated for use in ELISA, RIA, IH.

ELISA: Apparent binding constant from titration curves is 0.1 nM. Can be used as capture or reporter antibody for determination of collagen I or III in sandwich ELISA, but requires combination with collagen type-specific antibody for other function.

Immunohistochemistry: cryostat sections fixed with acetone or ethanol. Staining pattern similar to polyclonal antibody to interstitial collagens. Formaldehyde fixation is not recommended.

Not suitable for use on paraffin embedded sections or immunoblots.

Optimal working dilution must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Physical form

Format: Purified

Purified from murine ascites by PEG precipitation and Sepharose® CL-4B chromatography. Lyophilized Powder.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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