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  • MAB13458 - Anti-MMP-9 Antibody, clone 9D4.2

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MAB13458 Sigma-Aldrich

Anti-MMP-9 Antibody, clone 9D4.2

clone 9D4.2, Chemicon®, from mouse

Synonym: Gelatinase B, 92 kDa Type IV Collagenase

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, MF-MN, Primary Antibodies
clone   9D4.2, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG2a
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_004994.2
UniProt accession no.   P14780
Gene Information   human ... MMP9(4318)

Description

Specificity

Human MMP-9 pro and active forms.

Immunogen

Purified Human MMP-9 (Gelatinase B)

Application

Detect MMP-9 using this Anti-MMP-9 Antibody, clone 9D4.2 validated for use in ELISA & WB.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Western blot: 1:200- 1:1000

Immunocytochemistry: Not Tested

ELISA: 1:200-1:1000

Immunohistochemistry: Not Tested

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified

Purified immunoglobulin. Liquid in PBS containing 0.01% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6/12 months.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1150

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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