• USA Home
  • MAB1501-AF647 - Anti-Actin Antibody, clone C4, Alexa Fluor 647 conjugate

EMAIL THIS PAGE TO A FRIEND
MAB1501-AF647 Sigma-Aldrich

Anti-Actin Antibody, clone C4, Alexa Fluor 647 conjugate

clone C4, from mouse, ALEXA FLUOR® 647

Synonym: Actin, alpha skeletal muscle, Alpha-actin-1, Actin

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Primary Antibodies
conjugate   ALEXA FLUOR® 647
clone   C4, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
species reactivity   human, human
species reactivity (predicted by homology)   chicken (based on 100% sequence homology)
shipped in   wet ice
isotype   IgG2bκ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_001026234.1
UniProt accession no.   P68139
Gene Information   human ... ACTA1(58)

Description

General description

Actins are ubiquitous eukaryotic proteins that serve as a multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, trascriptional regulation and vesicle trafficking. These funstions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do not show spatial, temporal and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Beta and gamma-actins, also known as cytoplasmic actins, are highly conserved in higher animals and are predominantly expressed in non-muscle cells where they control cell structure. Exocytosis, and motility. They are nearly identical proteins and differ only in four amino acids at the N-terminal region. The other four actin isoforms are typically found in specific adult muscle tissue types. Alpha-cardiac and alpha-skeletal actins are expressed in striated cardiac and skeletal muscles, respectively. Alpha and gamma actins are primarily found in vascular smooth muscle and enteric smooth muscles, respectively. It has been shown that under calcium-bound conditions, beta-actin exhibits more dynamic behavior than gamma-actin with higher rates of polymerization and depolymerization. Also, beta- and gamma-actins can readily copolymerize, and the resulting filaments exhibit polymerization and depolymerization rates that vary depending on the ration of beta- to gamma-actin (Lessard, JL.,et al.(1988). Cell Motility Cytoskeleton 10(3); 349-362.

Specificity

To date, all animal species and cell types with an actin form react by indirect immunofluorescence or immunoblot, including plant actin.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Actin in NIH/3T3 cells.
The unconjugated version of this antibody (Cat. No. MAB1501) has been demonstrated to perform in WB, ELISA, ICC, IF, IHC, IH(P).

This Anti-Actin Antibody, clone C4, Alexa Fluor® 647 conjugate is validated for use in Immunocytochemistry for the detection of Actin.

Target description

40 kDa observed

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Actin in HeLa cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?