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MAB1510 Sigma-Aldrich

Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS)

This Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS) is validated for use in IH(P), IP, WB for the detection of Ubiquitin.

Synonym: Ubiquitin, ubiquitin-CEP80

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, U1-UG
clone   Ubi-1 (042691GS), monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable (paraffin)
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse, rat, human
shipped in   dry ice
antibody form   ascites fluid
isotype   IgG1κ
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_002954.3
UniProt accession no.   P62988

Description

General description

Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer′s disease, Lewy bodies seen in Parkinson′s disease, and Pick bodies found in Pick′s disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.

Specificity

Expected to cross-react with Bovine, Chicken, and Drosophila.

Reactivity with other species has not been confirmed.

Ubiquitin (76 amino acid, 8.5 kDa) both conjugated and unconjugated. Shows staining of tangle material in Alzheimer affected brain, and also stains other pathological inclusions such as Lewy bodies (in Parkinson’s disease) and Pick bodies (in Pick’s disease).

Immunogen

Purified ubiquitin (bovine RBC) covalently coupled to KLH using glutaraldehyde.

Application

Immunohistochemistry (frozen or paraffin embedded sections):
1:250-1:1,000 dilution from a previous lot was used.

Electron Microscopy:
1:250-1:1,000 dilution from a previous lot was used.

Immunoprecipitation:
(Liang, 1995; Oberdorf, 1999)
Note: Suggested blocking agent is bovine serum albumin.

Immunoblotting:
1:250-1:1,000. The sensitivity of the assay can be enhanced by autoclaving the blot (Swerdlow, 1986).

Optimal working dilutions must be determined by end user.

Research Category
Protein Trafficking

Neuroscience

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Target description

8.5 kDa

Physical form

Mouse monoclonal IgG1κ in buffer containing containing 10 mM sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquotes from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis:
1:500 dilution of this lot detected UBIQUITIN on 10 μg of Jurkat3 lysates.

Linkage

Replaces: 04-454

Analysis Note

Control
Alzheimer′s diseased brain.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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