• USA Home
  • MAB1572 - Anti-Parvalbumin Antibody

EMAIL THIS PAGE TO A FRIEND
MAB1572 Sigma-Aldrich

Anti-Parvalbumin Antibody

Anti-Parvalbumin Antibody is an antibody against Parvalbumin for use in IC, IH(P) & WB.

  •  eCl@ss 32160702

Purchase

Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µL (MAB1572)
isotype   IgG1
gene symbol   PVALB(5816)
trade name   Chemicon
brand family   Chemicon
format   Ascites
molecular weight   12 kDa
application(s)   Immunocytochemistry
  Immunohistochemistry (Paraffin)
  Western Blotting
clone   Monoclonal Antibody
  PARV-19
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
host   Mouse
species reactivity   Bovine
  Canine
  Feline
  Fish
  Frog
  Goat
  Human
  Mouse
  Pig
  Rabbit
  Rat
NCBI accession no.   NM_002854.2
UniProt accession no.   P20472
gene symbol   D22S749
  MGC116759
  parvalbumin
shipped in   dry ice
Quality Assurance   Routinely evaluated by Western Blot on mouse brain lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected Parvalbumin on 10 μg of mouse brain lysate.

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Brain tissue, Cultured neurons

Application

Immunohistochemistry:
A previous lot was used by indirect immunoperoxidase at 1:1,000-1:2,000 dilution. The antibody works on formalin fixed, paraffin embedded tissue sections of rat cerebellum from a previous lot. Fixation does not appear to affect MAB1572’s ability to detect parvalbumin.

Immunocytochemistry:
A previous lot of this antibody was used on cultured neurons.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

General description

Parvalbumin is a calcium binding albumin protein. It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is localised in fast-contracting muscles, where its levels are highest, and in the brain and some endocrine tissues. Parvalbumin is present in GABAergic interneurons in the nervous system, predominantly expressed by chandelier and basket cells in the cortex.

Immunogen

Parvalbumin purified from frog muscle

Specificity

Parvalbumin. The antibody will react with parvalbumin from brain and muscle. By immunoblot it recognizes a protein of 12 kDa. The antibody is directed against an epitope at the first Ca+2-binding site and specifically stains the Ca+2 -bound form of parvalbumin.

Physical form

Ascites mouse monoclonal IgG1 liquid

Unpurified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Making Headway
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?