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  • MAB1592 - Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1

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MAB1592 Sigma-Aldrich

Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, NE-NI, Primary Antibodies
clone   NP1, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  radioimmunoassay: suitable
  western blot: suitable
species reactivity   bovine, rat, human, chicken
shipped in   dry ice
antibody form   ascites fluid
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_021076.2
UniProt accession no.   P07197
  P12036

Description

General description

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

Specificity

Recognizes the KSP repeats of the phosphorylated forms of neurofilament-M (NF-M) and neurofilament-H (NF-H), because NF-L does contain a few of these Lysine-serine-PO4-proline repeats some cross reactivity to NF-L may be seen at very high antibody concentrations.

Immunogen

Bovine neurofilaments

Epitope: phosphorylated

Application

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Western blot : recognizes, 200kDa, and 150kDa NF-H and NF-M bands primarily. Staining is removed with phosphatase treatment. Because NF-L also contains lysine-serine PO4 sites, NP1 may cross react with NF-L as well, but this has not be substantiated in traditional westerns.

Immunohistochemistry. Works on paraffin embedded, formalin fixed and frozen acteone fixed tissue sections. 1:50-1:500 for immunofluorescence.

Immunocytochemistry on PC12, TERA-2 and other neuronal lines.

FACS ELISA/RIA

Optimal working dilutions must be determined by end user.

Physical form

Liquid. Contains no preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Linkage

Replaces: 04-1061

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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