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MAB1622 Sigma-Aldrich

Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6

Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6 is an antibody against Spectrin alpha chain (nonerythroid) for use in IH, IP, WB, ChIP.

Synonym: (ALPHA)II-SPECTRIN, Alpha-II spectrin, Fodrin alpha chain, Spectrin, non-erythroid alpha chain, non-erythrocytic spectrin alpha, spectrin, alpha, non-erythrocytic 1 (alpha-fodrin), Fodrin α

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SP-SS
clone   AA6, monoclonal
biological source   mouse
application(s)   ChIP: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   pig, rat, human, mouse, rat, rabbit, chicken, rabbit, pig, guinea pig, human, chicken, guinea pig, mouse
species reactivity (predicted by homology)   mammals
shipped in   wet ice
isotype   IgG1
antibody product type   primary antibodies
mfr. no.   Chemicon®
suitability   not suitable for immunohistochemistry (Paraffin)
NCBI accession no.   NM_003127.2
UniProt accession no.   Q13813
Gene Information   human ... SPTAN1(6709)

Description

General description

Non-erythroid Spectrin, also known as Fodrin, plays a role in stabilizing membrane structures, maintaining cell shape and linking the cytoskeleton to plasma membranes or intracellular vesicles. As one of the primary targets cleaved by activated caspases during apoptosis, the full-length ~240 kDa protein can be cleaved to yield an N-terminal product of ~150 kDa, and C-terminal products of ~120 and ~35 kDa.

Specificity

Specific for the 240, 150, and 120 kDa forms of alpha-spectrin/alpha-fodrin molecule of all mammalian nonerythroid cells and chicken alpha-spectrin.

Wide-mammalian reactivity is expected.

Immunogen

Chicken red blood cell membranes purified by hypotonic lysis and mechanical enucleation.

Application

Immunohistochemistry (IFA):
A 1:200-1:400 dilution of a previous lot was used in immunohistochemistry.
Does not work on paraffin sections.

Optimal working dilutions must be determined by end user.

Chromatin Immunopreciptiation:
A previous lot of this antibody was used to immunoprecipitate Spectrin Alpha from chromatin-associated protein extracts derived from normal human, HeLa, FA-A, and transduced FA-A cells (McMahon, L.W., et al. (1999). J. Biological Chem. 274:32904-32908).

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Target description

~240 kDa full length product, ~150 kDa N-terminal cleaved product and ~120 kDa C-terminal product may be seen in varing amounts in lysates, depending on condition. The smaller sizes represent break down products seen during apoptosis.

Physical form

Format: Purified

Protein A Purfied

Purified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.09% sodium azide.

Storage and Stability

Stable for 12 months at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot on staurosporine-treated HeLa lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected N-, C- terminal Spectrin alpha/Fodrin alpha on 10 μg of staurosporine treated HeLa lysates.

Analysis Note

Control
HeLa cells treated with staurosporine

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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