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  • MAB1628 - Anti-Myosin Antibody, slow muscle, clone NOQ7.5.4D

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MAB1628 Sigma-Aldrich

Anti-Myosin Antibody, slow muscle, clone NOQ7.5.4D

Anti-Myosin Antibody, slow muscle, clone NOQ7.5.4D is an antibody against Myosin for use in RIA, WB, IH.

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, MUE-MZ, Primary Antibodies
clone   NOQ7.5.4D, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
  radioimmunoassay: suitable
  western blot: suitable
species reactivity   feline, human, rat
shipped in   wet ice
isotype   IgG
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_000257.2
UniProt accession no.   P12883

Description

Specificity

Slow myosin heavy chain. Clearly identifies Type 1 fibers. Within skeletal muscle MAB1628 is specific for slow myosin heavy chain in a wide variety of species. It reacts strongly with rat and feline slow myosin heavy chain. MAB1628 also identifies beta (slow) myosin heavy chain in heart ventricles.

Immunogen

Epitope: slow muscle

Myosin purified from myofibrils isolated from histochemically mixed human skeletal muscle.

Application

Immunohistochemistry: frozen and formalin fixed sections.

Immunoblotting

RIA

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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