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  • MAB1682 - Anti-Actinin α Antibody, clone AT6/172

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MAB1682 Sigma-Aldrich

Anti-Actinin α Antibody, clone AT6/172

ascites fluid, clone AT6/172, Chemicon®

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Primary Antibodies
clone   AT6/172, monoclonal
biological source   mouse
application(s)   immunofluorescence: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human
shipped in   dry ice
antibody form   ascites fluid
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_001102.2
UniProt accession no.   P12814
Gene Information   human ... ACTN1(87)

Description

Specificity

Human alpha actinin. This antibody recognizes an epitope in the N-terminal region of alpha-actinin, probably between residues 1-24.

Immunogen

Epitope: alpha

Human platelet protein

Application

Anti-Actinin Antibody, α, clone AT6/172 detects level of Actinin & has been published & validated for use in IF, IP & WB.

Immunoblotting: ~100kDa on SDS reducing gels

Immunoprecipitation

Immunofluorescence: methanol fixed cells

Immunohistochemistry



Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Physical form

Liquid

Storage and Stability

Maintain frozen at -20°C for up to 12 months in undiluted aliquots. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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