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  • MAB1912 - Anti-Procollagen Type I Antibody, NT, clone M-58

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MAB1912 Sigma-Aldrich

Anti-Procollagen Type I Antibody, NT, clone M-58

culture supernatant, clone M-58, Chemicon®

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Miscellaneous, Primary Antibodies More...
clone   M-58, monoclonal
biological source   rat
application(s)   immunohistochemistry: suitable (paraffin)
species reactivity   human
should not react with   rat, mouse, hamster
antibody form   culture supernatant
isotype   IgG2α
Quality Level   100
mfr. no.   Chemicon®
NCBI accession no.   NM_000088.3
UniProt accession no.   P02452
Gene Information   human ... COL1A1(1277)

Description

Specificity

Recognizes Human pro-collagen I. MAB1912 labels the amino-terminal pro-peptide.

Application

Detect Procollagen Type I using this Anti-Procollagen Type I Antibody, N-terminus, clone M-58 validated for use in IH(P).

Immunohistochemistry: 1:10,000-1:50,000 (fresh frozen tissue sections.)

Suitable for formaldehyde/formalin fixed paraffin sections, suggested starting dilution 1:100-1:1000, after treatment with 1% trypsin, 20 minutes at room temperature. Does not stain mature collagen fibers in tissue. Localized intracellularly in cells producing pro-collagen I. Not recommended for Western Blots Optimal working dilutions must be determined by end user.

Analysis Note

Control
Skin tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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