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MAB1920-C Sigma-Aldrich

Anti-Laminin γ1 Antibody, clone 2E8 (Ascites Free)

Synonym: Laminin subunit gamma-1, Laminin B2 chain, Laminin-1/-2/-3/-4/-6/-7/-8/-9/-10/-11 subunit gamma, S-laminin subunit gamma, S-LAM gamma

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Primary Antibodies More...
application(s)   ELISA: suitable
  immunocytochemistry: suitable
  immunofluorescence: suitable
  western blot: suitable
biological source   mouse
clone   2E8, monoclonal
  monoclonal
isotype   IgG2aκ
shipped in   dry ice
species reactivity   mouse, feline, rat, human
gene symbol   LAMB2(3913)
  LAMC1(3915)

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

~200 kDa observed. Uncharacterized band(s) may appear in some lysates.

Application

Immunofluorescence Analysis: A representative lot detected laminin γ1 immunoreactivity in the extracellular matrix fractones by fluorescent immunohistochemistry using human and mouse brain cryosections (Kerever, A., et al. (2007). Stem Cells. 25(9):2146-2157).
Immunofluorescence Analysis: A representative lot immunostained the seminiferous cord basement membrane and vasculature in frozen rat testis cryosections by fluorescent immunohistochemistry (Johnson, K.J., et al. (2007). Biol. Reprod. 77(6):978-989).
Immunofluorescence Analysis: A representative lot detected Laminin γ1 immunoreactivity in unfixed frozen cat muscle sections by fluorescent immunohistochemistry (O′Brien, D.P., et al. (2001). J.Neurol. Sci. 189(1-2):37-43).
Immunofluorescence Analysis: A representative lot immunostained the trophoblast and the blood vessel basement membranes in frozen human placenta tissue sections by fluorescent immunohistochemistry (Engvall, E., et al. (1986). J. Cell Biol. 103(6 Pt 1):2457-2465).
Immunocytochemistry Analysis: A representative lot detected the laminin γ1 immunoreactivity at the substratum-attached surface of formaldehyde-fixed, Triton X-100-permeablized rat lung aveolar epithelial cells (AECs) by fluorescence immunocytochemistry (Jones, J.C., et al. (2005). J. Cell Sci. 118(Pt 12):2557-2566).
Western Blotting Analysis: Representative lots detected the ~200 kDa target band using rat lung aveolar epithelial cell (AEC) lysates, human amnion extracts, and rat yolk sac tumors (Jones, J.C., et al. (2005). J. Cell Sci. 118(Pt 12):2557-2566; Engvall, E., et al. (1986). J. Cell Biol. 103(6 Pt 1):2457-2465).
ELISA Analysis: A representative lot detected the binding of human laminin-10 (α5β1γ1) to immobilized α-dystroglycan and heparin by ELISA (Ido, H., et al. (2004). J. Biol. Chem. 279(12):10946-10954).
ELISA Analysis: A representative lot detected laminin γ1 subunit in human and rat, but not mouse, laminin preparations by ELISA (Engvall, E., et al. (1986). J. Cell Biol. 103(6 Pt 1):2457-2465).
Immunohistochemistry Analysis: A representative lot detected Laminin γ1 immunoreactivity in the perineurium of the perineurial cell basement membrane (PCBM) layers in paraffin-embedded human sural nerve sections (Hill, R.E., and Williams, R.E. (2002). J. Anat. 201(2):185-192).
Immunoprecipitation Analysis: A representative lot immunoprecipitated laminin from the culture medium of human choriocarcinoma (JAr) cells (Engvall, E., et al. (1986). J. Cell Biol. 103(6 Pt 1):2457-2465).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

General description

Laminin subunit gamma-1 (UniProt P11047; also known as Laminin B2 chain, Laminin-1/-2/-3/-4/-6/-7/-8/-9/-10/-11 subunit gamma, S-laminin subunit gamma, S-LAM gamma) is encoded by the LAMC1 (also known as LAMB2) gene (Gene ID 3915) in human. Laminins are basement membrane, extracellular matrix glycoproteins composed of alpha, beta, and gamma heterotrimeric chains. Different combinations of five alpha chains, three beta chains, and three gamma chains form the 15 known laminin heterotrimers, nine of which contain the gamma-1 chain: Laminin-1/EHS (α1β1γ1), Laminin-2/Merosin (α2β1γ1), Laminin-3/S-laminin (α1β2γ1), Laminin-4/S-merosin (α2β2γ1), Laminin-6/K-laminin (α3β1γ1), Laminin-7 (α3β2γ1), Laminin-8 (α4β1γ1), Laminin-9 (α4β2γ1), Laminin-10 (α5β1γ1), Laminin-11 (α5β2γ1). The gamma chain is initially produced with a signal peptide (a.a. 1-33), the removal of which yields the 1576-amino acid mature protein (a.a. 34-1609) with 11 EGF-like domains and a Laminin IV type A domain. Laminins are associated with type IV collagen networks via entactin, fibronectin, and perlecan. They also bind to cell membranes through integrin receptors and other plasma membrane molecules, such as the dystroglycan glycoprotein complex and Lutheran blood group glycoprotein. Laminins play important roles in cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype and survival.

Immunogen

Epitope: Center of the laminin cross.

Human placenta laminin pepsin digest.

Other Notes

Concentration: Please refer to lot specific datasheet.

Quality

Evaluated by Western Blotting in human heart tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Laminin γ1 in 10 µg of human heart tissue lysate.

Specificity

Detects the laminin γ1 component of Laminin-1/EHS (α1β1γ1), Laminin-2/Merosin (α2β1γ1), Laminin-3/S-laminin (α1β2γ1), Laminin-4/S-merosin (α2β2γ1), Laminin-6/K-laminin (α3β1γ1), Laminin-7 (α3β2γ1), Laminin-8 (α4β1γ1), Laminin-9 (α4β2γ1), Laminin-10 (α5β1γ1), Laminin-11 (α5β2γ1).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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