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MAB1944-C Sigma-Aldrich

Anti-Collagen VI alpha-3 Antibody, clone 3C4, Ascites Free

culture supernatant, clone 3C4, from mouse

Synonym: Collagen alpha-3(VI) chain, Collagen VI alpha-3 polypeptide

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CO-CP, Primary Antibodies
clone   3C4, monoclonal
biological source   mouse
application(s)   electron microscopy: suitable
  flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  immunoprecipitation (IP): suitable
species reactivity   human, human, human
shipped in   dry ice
antibody form   culture supernatant
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_004360
UniProt accession no.   P12111
Gene Information   human ... COL6A3(1293)

Description

General description

Collagen alpha-3(VI) chain (UniProt P12111; also known as Collagen VI alpha-3 polypeptide) is encoded by the COL6A3 gene (Gene ID 1293) in human. Type VI collagen is an extracellular matrix (ECM) component present in virtually all connective tissues, including cartilage, bone, tendon, muscles and cornea, where it forms microfibrils in close association with basement membranes. In addition to anchoring the basement membrane to the pericellular matrix in muscle, research also indicates a role for collagen VI in cell signaling and cell migration. The basic structural unit of collagen VI is a heterotrimer composed of the alpha-1(VI), alpha-2(VI), and alpha-3(VI) chains (encoded by the COL6A1, COL6A2, and COL6A3 genes, respectively). The α1(VI) and α2(VI) chains are similar in size and domain structure, they contain a 335- or 336-amino acid triple helix region that is characteristic of all collagens. Flanking the triple helix are domains homologous to the A-type domains found in von Willebrand factor (VWA domains). α1(VI) and α2(VI) contain one VWA domain N-terminal to the triple helix (N1) and two VWA domains C-terminal of the helix (C1 and C2). The α3(VI) chain, on the other hand, is much larger with 10 N-terminal (N1–N10) and two C-terminal VWA domains (C1 and C2), and several other types of identifiable domains in the C terminal region (C3–C5). Mutations in the COL6A1, COL6A2, and COL6A3 genes are known causes of Ullrich congenital muscular dystrophy (UCMD) and Bethlem myopathy (BM). Three additional type VI collagen chains have been reported in 2008 (α4(VI), α5(VI) and α6(VI) chains encoded by COL6A4, COL6A5, and COL6A6, respectively).

Immunogen

Purified human collagen VI.

Application

This Anti-Collagen VI alpha-3 Antibody, clone 3C4, Ascites Free is validated for use in Immunohistochemistry (Paraffin), Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Electron Microscopy for the detection of Collagen VI alpha-3.

Target description

343.7/321.4/113.2/278.2/134.7 kDa (isoform 1/2/3/4/5 pro-form) and 340.8/318.5/110.4/275.3/131.8 kDa (isoform 1/2/3/4/5 mature form) calculated

Quality

Evaluated by Immunohistochemistry in human breast tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected collagen VI alpha-3 chain immunoreactivity in human breast tissue section.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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