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  • MAB1957Z - Anti-Integrin β3 Antibody, clone 25E11, azide free

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MAB1957Z Sigma-Aldrich

Anti-Integrin β3 Antibody, clone 25E11, azide free

clone 25E11, Chemicon®, from mouse

Synonym: CD61, MAB1957

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, IN-IP, Primary Antibodies
clone   25E11, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
species reactivity   human
shipped in   wet ice
isotype   IgM
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_000212.2
UniProt accession no.   P05106
Gene Information   human ... ITGB3(3690)

Description

General description

The integrin family of cell adhesion receptors consists of several membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented.
Platelet membrane glycoprotein Integrin beta3 or CD61 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets.

Specificity

Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). The protein detectable is a complex of CD41 and CD61. Ligands are fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The monocytoid cell line U937 is highly positive.

(original paper claimed IgG2ak)

Immunogen

Normal blood mononuclear cells activated by mixed lymphocyte reaction and cultured for 10 days in medium containing IL-2.

Application

Detect Integrin β3 using this Anti-Integrin β3 Antibody, clone 25E11, azide free validated for use in FC, IP & IC.

Immunoprecipitation

Immunocytochemistry (acetone fixation required).

Flow cytometry

Inhibits platelet aggregation, 10 μg/mL

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Physical form

Format: Purified

In buffer of 0.01M phosphate, 0.15M sodium chloride, pH 7.1, containing no preservatives.

Storage and Stability

Maintain at 2-8°C.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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