• USA Home
  • MAB1961Z - Anti-Integrin αVβ5 Antibody, clone P1F6, azide free

EMAIL THIS PAGE TO A FRIEND
MAB1961Z Sigma-Aldrich

Anti-Integrin αVβ5 Antibody, clone P1F6, azide free

Detect Integrin αVβ5 using this Anti-Integrin αVβ5 Antibody, clone P1F6, azide free validated for use in FC, IH.

Synonym: MAB1961

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, IN-IP, Primary Antibodies
clone   P1F6, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunohistochemistry: suitable
species reactivity   human, chicken
shipped in   wet ice
isotype   IgG1
antibody product type   primary antibodies
Quality Level   100
mfr. no.   Chemicon®
suitability   not suitable for immunohistochemistry (Paraffin)
NCBI accession no.   NM_002210.2
UniProt accession no.   P06756
Gene Information   human ... ITGAV(3685)

Description

General description

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha V chain. Alpha chain V undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Specificity

Reacts with the human integrin alphaVbeta5.

Immunogen

Human UCLA P3 cells (lung carcinoma)

Application

Inhibition of attachment of HT29 cells to vitronectin: >1:1,000

Inhibition of attachment of carcinoma and melanoma cells to vitronectin

Immunohistochemistry: for use on acetone or paraformaldehyde fixed tissue. (Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.)

Immunocytochemistry: for use on human carcinomas, normal epithelium and keratinocytes 1:1500 (Acetone Fixed Only)

Immunoprecipitation

FACS

EIA

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Physical form

Format: Purified

Protein A Purfied

Purified immunoglobulin in 0.01M phosphate buffer, pH 7.1, 0.15M NaCl containing no preservatives.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Epithelium, keratinocytes

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?