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  • MAB1973Z - Anti-Integrin α1 Antibody, clone FB12, azide free

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MAB1973Z Sigma-Aldrich

Anti-Integrin α1 Antibody, clone FB12, azide free

clone FB12, Chemicon®, from mouse

Synonym: CD49a, MAB1973

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, IN-IP, Primary Antibodies
clone   FB12, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  flow cytometry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_181501.1
UniProt accession no.   P56199
Gene Information   human ... ITGA1(3672)

Description

Specificity

Reacts with the I domain (Val151 - Ala364) of human alpha1 integrin (Fabbri, 1996). Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi, 1991). Does not recognize recombinant I domain of rat integrin alpha1.

Application

Detect Integrin α1 using this Anti-Integrin α1 Antibody, clone FB12, azide free validated for use in ELISA, FC, IH, IP, FUNC & WB.

Immunohistochemistry: 2 μg/mL, with paraformaldehyde fixation. Immunoblotting: 2 μg/mL

Immunoprecipitation: 1 μg/mL

FACS analysis: 2 μg/mL

ELISA: 0.5 μg/mL

Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri, 1996): 1-5 μg/mL

Optimal working dilutions must be determined by end user.

Target description

130 kDa

Physical form

Format: Purified

Purified immunoglobulin in 0.02M PB, pH 7.6, 0.25M NaCl.

Analysis Note

Control
Microvascular endothelium

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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