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  • MAB2023Z - Anti-Integrin β3 Antibody, clone B3A, azide free

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MAB2023Z Sigma-Aldrich

Anti-Integrin β3 Antibody, clone B3A, azide free

Synonym: CD61, MAB2023

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, IN-IP, Primary Antibodies
clone   B3A, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
antibody product type   primary antibodies
Quality Level   100
mfr. no.   Chemicon®
NCBI accession no.   NM_000212.2
UniProt accession no.   P05106
Gene Information   human ... ITGB3(3690)

Description

Specificity

Specific for human integrin beta 3 (other species not tested).

Immunogen

Human integrin alphaVbeta3 heterodimer isolated from placenta.

Application

Flow cytometry

Function-blocking of integrin beta3-mediated adhesion to ECM proteins.

Western blot (reducing conditions) Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Target description

110 kDa

Physical form

Format: Purified

Protein A Purfied

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Positive Control: U251 or D54 human cell lines. Membrane preps are recommended for western blots.
Human endothelium cells (expression of integrin alpha V beta 3 may need to be upregulated by phorbol esters stimulation). Skin (Basement Membrane)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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