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  • MAB2162 - Anti-Adrenoleukodystrophy Protein Antibody, a.a. 495-648, clone 1AL-2B4

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MAB2162 Sigma-Aldrich

Anti-Adrenoleukodystrophy Protein Antibody, a.a. 495-648, clone 1AL-2B4

Anti-Adrenoleukodystrophy Protein Antibody, a.a. 495-648, clone 1AL-2B4 detects level of Adrenoleukodystrophy Protein & has been published & validated for use in ELISA, WB, IC, IH.

Synonym: ALDP

  •  eCl@ss 32160702

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Properties

Related Categories AD-AD, Alphabetical Index, Antibodies, Primary Antibodies
clone   1AL-2B4, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   dry ice
antibody form   ascites fluid
isotype   IgG1
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_000033.2
UniProt accession no.   P33897

Description

Specificity

Human adrenoleukodystrophy protein (ALDP). No cross-reactivity on mouse ALDP, mouse ALDRP and PMP 70. MAB2162 can be used in Western blot, immunofluorescence or immunohistochemical studies to analyze the ALD protein in cells and tissues from normal individuals or from patients with adrenoleukodystrophy (ALD) or Zellweger syndrome and related peroxisomal diseases, and provides an excellent marker for peroxisomes(Mosser et al., 1994). About 70% of ALD male patients have no detectable ALDP in fibroblasts or leukocytes. A similar proportion of carrier females may show mosaicism for the presence or absence of ALDP.

Immunogen

ALDP fragment from aa 495-648 as a fusion protein

Epitope: a.a. 495-648

Application

ELISA: 1:500-1:5,000

Western blot: 1:500-1:5,000

Immunohistochemistry: 1:500-1:5,000

Immunocytochemistry: 1:500-1:5,000

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Physical form

Ascites. Liquid, does not contain any preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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