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  • MAB2247 - Anti-Integrin β1 Antibody, clone 12G10

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MAB2247 Sigma-Aldrich

Anti-Integrin β1 Antibody, clone 12G10

Anti-Integrin β1 Antibody, clone 12G10 detects level of Integrin β1 & has been published & validated for use in ELISA, FC, IP, FUNC, CULT & WB.

Synonym: Integrin beta-1, CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µg (MAB2247)
application(s)   ELISA: suitable
  cell analysis: suitable (cell culture)
  flow cytometry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
biological source   mouse
clone   12G10, monoclonal
  monoclonal
isotype   IgG1κ
mfr. no.   Chemicon
shipped in   dry ice
species reactivity   human
gene symbol   ITGB1(3688)
  FNRB
  MDF2
  MSK12
  VLA-BETA
  VLAB

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

88 kDa

Analysis Note

Control
Tonsil, human skin tissue

Application

Flow cytometry: 1:25-1:50

ELISA : 10 μg/mL

Western Blot: known to react with human beta 1 integrin subunit under non-reduced conditions only {Mould, 1995}.

Stimulatory to alpha5beta1 interaction with fibronectin {Mould, 1995}.

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

General description

Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.

Immunogen

Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Specificity

MAB2247 recognizes the human beta1 integrin. This antibody has been shown to recognize a ligand-induced binding site, with antibody binding increasing in the presence of a fibronectin ligand. This antibody also enhances alpha 5 - beta 1 fibronectin interactions. MAB2247 does not cross-react with mouse or rat samples.

Physical form

Format: Purified

Protein A Purfied

Purified immunoglobulin. Liquid in phosphate buffer saline, with 0.1% sodium azide.

Storage and Stability

Maintain refrigerated at 2–8°C in undiluted aliquots for up one month. For long term storage maintain at -20°C for up to 12 months.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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