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  • MAB2617 - Anti-Kindlin-2 Antibody, clone 3A3

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MAB2617 Sigma-Aldrich

Anti-Kindlin-2 Antibody, clone 3A3

clone 3A3, from mouse

Synonym: Mitogen-inducible gene 2 protein, Pleckstrin homology domain-containing family C member 1, fermitin family homolog 2, kindlin 2, mitogen inducible gene 2 protein

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, KIA-KIT, Primary Antibodies
clone   3A3, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  western blot: suitable
species reactivity   human, bovine, rat, mouse, human
species reactivity (predicted by homology)   bovine (based on 100% sequence homology), mouse (98% sequence homology), rat (98% sequence homology)
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_001128471
UniProt accession no.   Q96AC1
Gene Information   human ... FERMT2(10979)

Description

General description

The Kindlin family is a group of focal adhesion proteins that have become a point of interest because of their ability to bind and activate integrins. Abnormalities in this ability have linked Kindlin to several diseases, both inherited and acquired, such as Kindler syndrome, cancer, and leukocyte adhesion deficiency. The isoform Kindlin-2 specifically participates in the recruitment of migfilin protein to focal adhesion sites for cell-ECM interactions, coordinates the manufacture of actin and modifications to cell shape. It is also actively involved in connecting ECM adhesion sites and the actin cytoskeleton (Shi, 2007). Kindlin-2 is pervasively expressed throughout tissue.

Specificity

The antibody recognizes Kindlin-2.

Immunogen

Epitope: Unknown

GST-tagged recombinant protein corresponding to human Kindlin-2.

Application

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-Kindlin-2 Antibody, clone 3A3 is validated for use in WB, IC for the detection of Kindlin-2.

Target description

~ 70 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Kindlin-2 in 10 µg of HeLa cell lysate.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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