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  • MAB2625 - Anti-XB130 Antibody, clone 12

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MAB2625 Sigma-Aldrich

Anti-XB130 Antibody, clone 12

clone 12, from mouse

Synonym: Actin filament associated protein 1-like 2, AFAP1-like protein 2

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, X
clone   12, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  activity assay: suitable
  immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG2aκ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_115939.1
UniProt accession no.   Q8N4X5
Gene Information   human ... AFAP1L2(84632)

Description

General description

XB130 is a 130 kDa adaptor protein and Src kinase substrate, structurally similar to actin-filament-associated protein. Recent evidence indicates tyrosine 54 of XB130 as the major target of RET/PTC-mediated phosphorylation and a critical binding site for the SH2 domains of p85alpha. Importantly, downregulation of XB130 in TPC1 papillary thyroid cancer cells, harboring the RET/PTC1 kinase, strongly reduced Akt activity without altering ERK1/2 phosphorylation, and concomitantly inhibited cell-cycle progression and survival in suspension. XB130 is a novel substrate of the RET/PTC kinase that links RET/PTC signaling to PI 3-kinase activation, and thereby plays an important role in sustaining proliferation and survival of thyroid tumor cells, (Lodyga, 2009).

Specificity

The antibody recognizes XB130.

Immunogen

Epitope: Unknown

His-tagged full length recombinant protein corresponding to human XB130.

Application

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

This Anti-XB130 Antibody, clone 12 is validated for use in WB, IP, IC, ELISA, EA for the detection of XB130.

Target description

The calculated MW is ~ 91 kDa However, it is usually observed as 110-130 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot on A549 cell lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected XB130 on 10 µg of A549 cell lysate.

Analysis Note

Control
A549 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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