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  • MAB312R - Anti-A2B5 Antibody, clone A2B5-105

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MAB312R Sigma-Aldrich

Anti-A2B5 Antibody, clone A2B5-105

Synonym: Neuron Cell Surface Antigen

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories A1-AB, Alphabetical Index, Antibodies, Primary Antibodies
clone   A2B5-105, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable
species reactivity (predicted by homology)   mammals
shipped in   dry ice
isotype   IgM
antibody product type   primary antibodies
Quality Level   100
mfr. no.   Chemicon®

Description

General description

A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors and neuroendocrine cells. The A2B5 antigen is present in type 2 astrocytes but absent in type 1 astrocytes, allowing for the differentiation of these different types of cells. A2B5 antibody has been used in flow cytometry and immunohistochemistry experiments to stain type II astrocytes, cells involved in gliogenesis and cells committed to the oligodendrocyte lineage.

Specificity

Recognizes an epitope common to silaogangliosides and sulfatides from the plasma membranes of neurons, and neuroendocrine and glial cells. Anti-A2B5 has been used to specifically stain:

Neurofibrillary tangles in the post-mortem brain tissue of patients with a confirmed diagnosis of Alzheimer′s disease (Majocha et al., 1989)
Neurons of chicken retina, brain, spinal cord, and dorsal root ganglia (Schnitzer & Schachner, 1982; Eisenbarth et al., 1979).
Rat striatal nerve terminals [synaptosomes (Wolf & Kapatos, 1989)].
Neuroblastoma cells in man (Oppedal et al., 1989; Coakham et al., 1985).
Rat insulinoma cells and pancreatic islets cells (Shimizu et al., 1983; Bartholomeusz et al., 1989).
Human and rat thymic epithelial cells (Haynes et al., 1988).
Cells involved in gliogenesis (Bottenstein et al., 1988; Suzumura & Slerberg, 1989).
Oligodendrocytes and astrocytes, type II (Dubois-Dalcq et al., 1990).

Immunogen

Embryonic chicken retinal cells

Application

Immunocytochemistry: 5-10 μg/mL Immunohistochemistry: 5-10 μg/mL (Levison & McCarthy, 1989)

Complement-mediated cytotoxicity (Eisenbarth et al., 1979)

Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Physical form

Ammonium Sulfate Precipitation

Format: Purified

Liquid. Buffer = PBS, pH 7.1 with 2 mg/mL BSA.

Storage and Stability

Maintain the lyophilizate frozen at -20°C. Store the reconstituted antibody solution at -20°C for 12 months in undiluted aliquots.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Type II astrocytes, human neural progenitors

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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