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  • MAB3242 - Anti-Smoothelin Antibody, clone R4A

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MAB3242 Sigma-Aldrich

Anti-Smoothelin Antibody, clone R4A

Detect Smoothelin using this Anti-Smoothelin Antibody, clone R4A validated for use in WB, IC, IH(P).

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Miscellaneous, Primary Antibodies More...
clone   R4A, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   canine, feline, hamster, chicken, human, monkey, pig
shipped in   dry ice
isotype   IgG1
Quality Level   100
mfr. no.   Chemicon®
NCBI accession no.   NM_006932.3
  NM_134269.1
  NM_134270.1
UniProt accession no.   P53814
Gene Information   human ... SMTN(6525)

Description

General description

Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cell with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin or vimentin. Smoothelin colocalizes with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 50 kDa isoform specific for visceral SMC, and an isoform with a molecular weight of approximately 100 kDa in vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.MAB3242 is useful to discriminate fully differentiated, contractile SMCs from proliferative SMCs. The distribution of smoothelin-positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Smoothelin is a useful tool in the evaluation of atherosclerotic lesions.

Specificity

MAB3242 reacts with smoothelin, a cytoskeletal protein exclusively found in smooth muscle cells. Reacts with the 59 kDa and 100 kDa protein corresponding to smoothelin by Western blot.

Immunogen

Cytoskeletal extract of chicken gizzard.

Application

Western blot

Immunocytochemistry

Immunohistochemistry on both frozen and paraffin embedded tissue sections. For paraffin sections, high temperature antigen retrieval in 10 mM citrate buffer (pH 6.0) is highly recommended (van der Loop, 1997).

Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified

Liquid in buffer (PBS) with 0.1% sodium azide.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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