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  • MAB324 - Anti-Neuron Specific Enolase Antibody, clone 5E2

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MAB324 Sigma-Aldrich

Anti-Neuron Specific Enolase Antibody, clone 5E2

clone 5E2, Chemicon®, from mouse

Synonym: NSE

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Antibodies for Neurobiology, Antibodies to Neural Proteins, Miscellaneous, Neuroscience,
Quality Level   100
biological source   mouse
clone   5E2, monoclonal
species reactivity   human
mfr. no.   Chemicon®
application(s)   ELISA: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  western blot: suitable
isotype   IgG2a
NCBI accession no.   NM_001975.2
UniProt accession no.   P09104
shipped in   wet ice

Description

Specificity

Human gamma neuron specific enolase. Recognizes a 45kD band corresponding to gamma NSE on immunoblots of human brain extracts.

Immunogen

Neuron-specific enolase from human brain.

Application

Anti-Neuron Specific Enolase Antibody, clone 5E2 detects level of Neuron Specific Enolase & has been published & validated for use in ELISA, WB, IC, IH(P).

Immunoblotting

ELISA

Immunohistochemistry. May be used on formalin-fixed, paraffin embedded sections.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Linkage

Replaces: CBL220

Physical form

Format: Purified

Liquid in PBS pH 7.4 containing 0.1% sodium azide.

Storage and Stability

Maintain at 2-8 °C for up to 12 months under sterile conditions.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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