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  • MAB329 - Anti-Synaptophysin Antibody, clone SP15

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MAB329 Sigma-Aldrich

Anti-Synaptophysin Antibody, clone SP15

ascites fluid, clone SP15, Chemicon®

Synonym: Major Synaptic Vesicle Protein p38

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SU-SY
clone   SP15, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   hamster, pig, rat, human
shipped in   dry ice
antibody form   ascites fluid
isotype   IgM
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_003179.2
UniProt accession no.   P08247
Gene Information   human ... SYP(6855)

Description

Specificity

Synaptophysin. Reacts with synaptophysin expressed in CHO cells.

Immunogen

Crude synaptic immunoprecipitate (Human)

Application

Immunohistochemistry at 1:200-1:5,000.

Formalin fixed (fixed <4 weeks), paraffin embedded

Frozen

4% Paraformaldehyde

Western blotting at 1:2,500-1:10,000.

ELISA at 1:500

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

This Anti-Synaptophysin Antibody, clone SP15 is validated for use in ELISA, WB, IH(P) for the detection of Synaptophysin.

Physical form

Liquid

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Linkage

Replaces: 04-1019

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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