• USA Home
  • MAB3303 - Anti-Collagen Type VI Antibody, clone VI-26

EMAIL THIS PAGE TO A FRIEND
MAB3303 Sigma-Aldrich

Anti-Collagen Type VI Antibody, clone VI-26

clone VI-26, Chemicon®, from mouse

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, CO-CP, Primary Antibodies
clone   VI-26, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunohistochemistry: suitable (paraffin)
species reactivity   human, rabbit
should not react with   rat
shipped in   dry ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
availability   not available in Japan
NCBI accession no.   NM_001845.4
UniProt accession no.   P02462
Gene Information   human ... COL4A1(1282)

Description

Specificity

Specifically reacts with the hCL(VI). This is a purified mouse monoclonal antibody to human type VI collagen. VI-26 does not react with denatured or reduced type VI collagen, although this antibody does recognize the the triple helix consisting of alpha1(VI), alpha 2(VI) alpha3(gamma). The epitope has not been mapped and the monoclonal is know not to react with collagens I, II, III, IV or V in native assays.

Application

Anti-Collagen Type VI Antibody, clone VI-26 detects level of Collagen Type VI & has been published & validated for use in ELISA, IH(P).

Immunohistochemistry on acetone fixed paraffin-embedded tissues. Formalin fixation is not recommended. Antigen recovery is treatment with 0.04% trypsin in 0.01M CaCl(2) 0.05M Tris-HCL pH 7.6, 37°C for 10 minutes before the non-specific peroxidase block.

EIA

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Physical form

Format: Purified

Liquid in 0.1 M sodium phosphate buffer, pH 7.0, containing 2% protease-free bovine Serum albumin.

Protein A Purfied

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Testis, connective tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?